In Vitro Pathogenesis Caused by Phytophthora cactorum and DNA Analysis of the Strawberry-Resistant Microplants with ISSR Markers

The soil pathogenic fungus Phytophthora cactorum causes the most dangerous diseases occurring in strawberry plantations—strawberry crown rot and leather rot. Modern biotechnology methods, e.g., vitro culture selection molecular diagnostics can be utilized of cultivars that are less susceptible or resistant to diseases. In this study, four microclones: ‘Elsanta’, ‘Feltar’, ‘Teresa’ ‘Plena SVdT’ against (Lebert Cohn) J. Schröt was carried out. Molecular analysis with inter simple sequence repeat (ISSR) markers also used evaluate genetic similarity selected plants. None analyzed microclones showed complete resistance factor, but there were plants all tested survived pressure pathogen. Results susceptibility significantly differentiated depended on microclone. ‘Feltar’ microclone had lowest disease, followed by ‘Elsanta’ higher susceptibility. highest disease. This differentiation linked observed at deoxyribonucleic acid (DNA) level between from microclones. Cluster revealed similar phytophthorosis, i.e., ‘Teresa’, appeared genetically similar. a different course phytophthorosis aforementioned microclones, being least them.